| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10536257 | Analytical Biochemistry | 2005 | 7 Pages |
Abstract
A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Arun K. Attri, Allen P. Minton,