Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10536298 | Analytical Biochemistry | 2005 | 8 Pages |
Abstract
The direct electron transfer of surface-confined horse heart cytochrome c (Cyt c) was achieved using COOH-terminated alkanethiolate-modified gold electrode. Later DNA was immobilized on the two-layer modified electrode. The quantitative determination of DNA was explored and the interaction between cytochrome c and DNA was studied. The binding site sizes were determined to be 15 bp per Cyt c molecule with double-stranded (ds) DNA and 30 nucleotides binding one Cyt c molecule with single-stranded (ss) DNA. At the dsDNA/Cyt c/MUA/Au electrode, the rate constant of oxidation electron transfer ks, ox = 1.59 Ã 10â3 cm sâ1 was obtained, at the ssDNA/Cyt c/MUA/Au electrode, the value was 2.43 Ã 10â3 m sâ1 when the scan rate was 1.0 V/s. The different electrodes were characterized with electrochemical quartz crystal microbalance and atomic force microscope.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
XiaoQin Ding, Jinhua Li, Jingbo Hu, Qilong Li,