Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10536324 | Analytical Biochemistry | 2005 | 11 Pages |
Abstract
Carbohydrate binding underlies many cell recognition events. Here, we describe a multiplexed glyco-bead array method for determining the carbohydrate-binding specificities of plant lectins using a bead-based flow cytometric analysis. N-glycans including high mannose, hybrid, and complex types and O-glycans from glycoproteins were immobilized on multiplexed beads, and the specificities of 13 kinds of sugar chains were monitored within 2Â h in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples and allows the reliable and simultaneous elucidation of sugar-binding properties under identical conditions.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kazuo Yamamoto, Seiichiro Ito, Fumiko Yasukawa, Yukiko Konami, Naoki Matsumoto,