| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10536826 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 9 Pages |
Abstract
⺠Crystal structure of a segment of the PKD2L1 cytoplasmic regulatory domain (CRD). ⺠Structure reveals a trimeric coiled coil in the predicted oligomerization region. ⺠Trimerization driven by central β-branched residues and stabilized by a salt bridge. ⺠Mutation of central residues converts high-affinity trimeric CRD to monomer. ⺠Monomer binds Ca2+ with WT-affinity, loses significant α-helicity upon ligand removal.
Keywords
HEPESpkd1l3HEKHEPES-buffered saline solutionPKD2L1PKD1CRDHBSSADPKDCC24-(2-hydroxyethyl)-1-piperazineethanesulfonic acidEDTAethylenediaminetetracetic acidTevautosomal dominant polycystic kidney diseasecircular dichroismendoplasmic reticulumTrimerizationMALDI-MSTobacco etch virushuman embryonic kidneyCoiled-coil
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Katrina L. Molland, Lake N. Paul, Dinesh A. Yernool,