| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10536830 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 7 Pages |
Abstract
⺠Direct determination of Km and Vmax from progress curves using 1H NMR spectroscopy. ⺠Applying Lambert W function analysis for fitting the experimental progress curves. ⺠NMR can easily detect and quantify in situ all reaction products and substrates. ⺠NMR allows the direct analysis of the stereochemistry of the products. ⺠NMR is a powerful tool to analyze Michaelis-Menten kinetics of enzyme reactions.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Franziska Exnowitz, Bernd Meyer, Thomas Hackl,