| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10536832 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 11 Pages |
Abstract
⺠Sequence analysis reveals that LRRLRRK2 contains 14 putative LRRs. ⺠A model of LRRLRRK2 was constructed using an LRR template assembly approach. ⺠Recombinant LRRLRRK2 was purified from E. coli. ⺠Chemical cross-linking studies revealed that LRRLRRK2 presents as a monomer. ⺠Clinical mutant forms of LRRLRRK2 did not influence its macromolecular properties.
Keywords
DTTBS3EGSIMACDLSGuHClrelative accessible surface areaIPTGIEXDDMbis(sulfosuccinimidyl) suberateSDSEDCSEC1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonaten-dodecyl-β-d-maltopyranosideEDTAethylene-diamine-tetra-acetic acidUltravioletSDS-polyacrylamide gel electrophoresisSDS-PAGEIsopropyl-β-d-thiogalacto-pyranosideIon exchangeleucine-rich repeatProtein purificationdithiothreitolcircular dichroismsodium dodecyl sulfateStokes radiusRASALuria BertaniHomology modelingMolecular weightDynamic Light ScatteringCross-linkingCHAPSSize exclusion chromatographyimmobilized metal ion affinity chromatographyGlutaraldehydeGuanidine hydrochloride
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Renée Vancraenenbroeck, Evy Lobbestael, Stephen D. Weeks, Sergei V. Strelkov, Veerle Baekelandt, Jean-Marc Taymans, Marc De Maeyer,