| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10536859 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 11 Pages |
Abstract
⺠We engineer aspartate aminotransferase to change substrate and reaction specificity. ⺠Substitutions (I33Q/Y214Q/R280Y) generate a cysteine sulfinate desulfinase. ⺠Desulfinase to transaminase activity fold enhancement in the mutant enzyme is 105. ⺠X-ray structures of engineered enzyme in PLP, PMP and alanyl-PLP forms solved. ⺠Mutated residues are present in naturally occurring desulfinases and decarboxylases.
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Authors
Francisco J. Fernandez, Dominique de Vries, Esther Peña-Soler, Miquel Coll, Philipp Christen, Heinz Gehring, M. Cristina Vega,