| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10537143 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2013 | 7 Pages |
Abstract
⺠An efficient approach to express and purify rLcn11 protein and C59A/C156A mutant. ⺠The first study of the physicochemical properties of rLcn11 protein. ⺠The recombinant rLcn11 protein forms dimer in neutral solution. ⺠The C59A/C156A rLcn11 processes multiple binding sites for hydrophobic ligand. ⺠A moderate binding affinity of C59A/C156A rLcn11 for binding ligand AMA.
Keywords
MALDI-TOFIPTGPheromone-binding proteinsMUPEGSOBPDLSPBPL-PGDSβ-Barrelisopropyl β-d-thiogalactosideDimercircular dichroismmatrix-assisted laser desorption ionization time-of-flightLuria-BertaniLipocalinlipocalin-type prostaglandin D synthaseLigand-bindingwild-typeDynamic Light Scatteringodorant-binding proteinDisulfide bond
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yina Gu, Qiang Liu, Peiyan Chen, Chenyun Guo, Yan Liu, Yufen Zhao, Yonglian Zhang, Donghai Lin,