| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10537169 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2013 | 7 Pages |
Abstract
⺠We cloned and expressed the mannanases of Streptomyces thermoluteus and S. lividans. ⺠Carbohydrate-binding modules enhanced mannanase activity, but not thermal stability. ⺠Binding of certain bivalent ions to mannanase enhanced thermal stability. ⺠Binding involved positive entropy change due to dehydration of protein-bivalent ion. ⺠Conformational change induced by certain bivalent ions was different among mannanases.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yuya Kumagai, Kayoko Kawakami, Misugi Uraji, Tadashi Hatanaka,