Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Ã resolution

PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 Å data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P62 or P64, with unit cell parameters a = b = 62.2, c = 39.9 Å. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.