| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10537385 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2011 | 10 Pages |
Abstract
⺠Crystallins form a broad mixture of cross-linked species of ~ 40 kDa in the water-soluble fractions of human lenses of all ages. ⺠Low molecular weight crystallin fragments were increasingly found in the water-insoluble lens fractions with age. ⺠Proteolysis of beaded filament proteins occurs very early in life. ⺠α-Crystallin interacts with intermediate filament proteins and their breakdown products under mild heat stress.
Keywords
NUSIEFPMFHRPPTMsMBPIFSBFSCWSCuSPVDFMIPbeaded filamentsNWsIgGCIDESICuIIPGimmobilized pH gradientWater-insolublePeptide mass fingerprintingCollision-induced dissociationimmunoglobulin Gposttranslational modificationsisoelectric focusingmatrix-assisted laser desorption/ionizationIntermediate filamentsAgingMass spectrometryLensMALDIWater-solublepolymerase chain reactionPCRhorseradish peroxidisemajor intrinsic proteinmaltose-binding proteinPolyvinylidene fluoridecrystallinselectrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shih-Ping Su, Jason D. McArthur, Roger J.W. Truscott, J. Andrew Aquilina,