| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10537389 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2011 | 9 Pages |
Abstract
⺠Construction of fully functional Cysteine-free (Cys-less) Elongation Factor Tu. ⺠Rapid-kinetics determination of rate constants governing nucleotide interaction. ⺠Effects on aminoacyl-tRNA binding properties. ⺠Highly conserved Cysteine 81 is not strictly required for EF-Ts and ribosome interaction. ⺠Cysteine 81 is involved in aminoacyl-tRNA binding through positioning of helix B.
Keywords
aminoacyl-tRNAaa-tRNAIPTGTPCKPEPTCAEF-Tutrichloroacetic acidsodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGEFluorescence resonance energy transferFRETisopropyl-β-d-thiogalactopyranosideRapid kineticsProtein designelongation Factor TuEvolutioninorganic phosphatephosphoenolpyruvateFluorescenceLuria–BertaniSingle molecule
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Evelina Ines De Laurentiis, Fan Mo, Hans-Joachim Wieden,