Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10537529 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2015 | 11 Pages |
Abstract
The lectins, a class of proteins that occur widely in animals, plants, fungi, lichens and microorganisms, are known for their ability to specifically bind to carbohydrates. Plant lectins can be classified into 12 families including the Galanthus nivalis agglutinin (GNA)-related lectin superfamily, which is widespread among monocotyledonous plants and binds specifically to mannose, a behavior that confers remarkable anti-tumor, anti-viral and insecticidal properties on these proteins. The present study characterized a mitogenic lectin from this family, called tarin, which was purified from the crude extract from taro (Colocasia esculenta). The results showed that tarin is a glycoprotein with 2-3% carbohydrate content, composed of least 10 isoforms with pIs ranging from 5.5 to 9.5. The intact protein is a heterotetramer of 47Â kDa composed of two non-identical and non-covalently associated polypeptides, with small subunits of 11.9Â kDa and large subunits of 12.6Â kDa. The tarin structure is stable and recovers or maintains its functional structure following treatments at different temperatures and pH. Tarin showed a complex carbohydrate specificity, binding with high affinity to high-mannose and complex N-glycans. Many of these ligands can be found in viruses, tumor cells and insects, as well as in hematopoietic progenitor cells. Chemical modifications confirmed that both conserved and non-conserved amino acids participate in this interaction. This study determined the structural and ligand binding characteristics of a GNA-related lectin that can be exploited for several different purposes, particularly as a proliferative therapeutic molecule that is able to enhance the immunological response.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
PatrÃcia R. Pereira, Harry C. Winter, Mauricio A. VerÃcimo, Jennifer L. Meagher, Jeanne A. Stuckey, Irwin J. Goldstein, Vânia M.F. Paschoalin, Joab T. Silva,