| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10537623 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2005 | 5 Pages |
Abstract
Pure honeybee venom samples were submitted to two-dimensional gel electrophoresis. A total of 49 excised spots were analyzed by mass spectrometry; 39 of them resulted in the identification of 6 different known bee venom proteins and of 3 proteins that have not been described in such samples before. The first new venom protein has a platelet-derived and vascular endothelial growth factor family domain, the second protein shows no homologies with any known protein and the third matches a hypothetical protein similar to major royal jelly protein 8.
Keywords
ESIMRJPPLA2IEFTOFMajor royal jelly protein2-DEIPGimmobilized pH gradientphospholipase A2ESTtwo-dimensional gel electrophoresisIUISproteome analysisisoelectric focusingExpressed Sequence Tagtime of flightHoneybeeVenomMass spectrometryMALDIHymenopteraliquid chromatographyelectrospray ionizationmatrix-assisted laser desorption ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nico Peiren, Frank Vanrobaeys, Dirk C. de Graaf, Bart Devreese, Jozef Van Beeumen, Frans J. Jacobs,