Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10537670 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 7 Pages |
Abstract
⺠Acetyl esterases display a distinct substrate selectivity profile. ⺠Novel fluorogenic ester substrates provide highly sensitive kinetic measurements. ⺠Two hydrophobic plasticity residues maintain this unusual substrate specificity. ⺠Substitution of these plasticity residues imparts broader specificity to TM0077. ⺠With broad specificity and high stability, TM0077 could be a valuable biocatalyst.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Matthew K. Hedge, Alexandra M. Gehring, Chinessa T. Adkins, Leigh A. Weston, Luke D. Lavis, R. Jeremy Johnson,