Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10537925 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2005 | 6 Pages |
Abstract
Human DPY-30-like is a homolog of C. elegans DPY-30. DPY-30 is an essential component of dosage compensation machinery and loss of dpy-30 activity results in XX-specific lethality. In XO animals, DPY-30 is required for developmental processes other than dosage compensation. In yeast, the homolog of DPY-30, Saf19p, functions as a member of histone 3 lysine 4 methylation complex, which is the key part of epigenetic developmental control. In this report, human DPY-30-like protein was overexpressed and purified with the goal of structure determination. It was crystallized at 291 K in hanging drops by the vapour diffusion technique from a precipitant solution consisting of (NH4)2SO4 (1.5-2.0Â M), Tris-HCl (0.1 M, pH 8.0). The crystal diffracted to 2.7 Ã
resolution at 100 K in-house and belongs to the space group P41212 or P43212 with unit-cell parameters of a = b = 74.5 Ã
, c = 87.0 Ã
, α = β = γ = 90.0°. The asymmetric unit contains two molecules with 49% solvent content. We also analyzed its biochemical and biophysical characterizations. Efforts are now under way to determine the molecular structure of the DPY-30-like. These studies will open a new avenue towards the structureâbased functional analysis of human DPY-30-like and dosage compensation machinery.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiuhua Dong, Yong Peng, Ying Peng, Feng Xu, Xiaojing He, Feng Wang, Xiaozhong Peng, Boqin Qiang, Jiangang Yuan, Zihe Rao,