| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10538719 | Food Chemistry | 2012 | 10 Pages |
Abstract
⺠β-Galactosidase from chick pea was purified 1840 fold with 12% recovery. ⺠It hydrolyses lactose with Km and Ea values to be 10 mM and 10.57 kcal moLâ1. ⺠Galactose was found to be competitive inhibitor. ⺠It was localised in germinated seeds with X-gal activity staining. ⺠Sequence alignment showed high similarity with other plant β-galactosidases.
Keywords
PDB5-bromo-4-chloro-3-indolyl-β-d-galactopyranosideX-GalCicer arietinumPMSFONPGpNPGDTTFPLCDMFLC–MS/MSN,N-dimethyl formamideBasic Local Alignment Search ToolBlastβ-galactosidaseSequence alignmentdithiothreitolMass spectrometryLiquid chromatography mass spectrometryfast performance liquid chromatographyPhenylmethanesulfonyl fluoridePurificationProtein Data BankGlycoprotein
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Devesh Kishore, Arvind M. Kayastha,