| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10538747 | Food Chemistry | 2012 | 5 Pages |
Abstract
⺠Optimum conditions for fruit bromelain activity on five substrates were determined. ⺠Optimum activity at 55 °C and pH 7.5 for azoalbumin and 6.5 for azocasein was obtained. ⺠Bromelain activity on casein and sodium caseinate shown optimum at 59 °C and pH of 7.7 and 6.5 respectively and pH 2.9 and 37 °C for haemoglobin. ⺠Azoalbumin and azocasein are the most recommended substrates for the enzyme evaluation due to their kinetic behavior. ⺠Results obtained show a new assay technique for the standardization of enzymes preparations and the measurement of enzymatic activities.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Carlos A. Corzo, Krzysztof N. Waliszewski, Jorge Welti-Chanes,