| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10538858 | Food Chemistry | 2012 | 7 Pages |
Abstract
⺠W/o microemulsions extract impurity proteins from β-galactosidase (β-gal) in one-step. ⺠Solubilisation studies determine microemulsion conditions for excluding β-gal. ⺠Mixtures contained β-gal and impurity protein (β-Lg) with similar pI but lower MW. ⺠Microemulsions with [buffer] >40 mM and added KCl extract β-Lg, expel purified β-gal. ⺠At 100 mM [KCl], process yields virtually pure β-gal with 92% activity recovery.
Related Topics
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Authors
Bekir G. Mazı, Haluk Hamamcı, Stephanie R. Dungan,