Comparisons of α-amylase inhibitors from seeds of common bean mutants extracted through three phase partitioning

► TPP technology can be used to purify the α-amylase inhibitor protein form common bean seeds. ► NaN3-induced mutation affects the activity of α-amylase inhibitor on common bean seeds. ► Significant variations in the activity of α-amylase inhibitor were observed with the mutant SA-05 had the highest activity amongst the tested accessions.