| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10539877 | Food Chemistry | 2013 | 10 Pages |
Abstract
Grape seed extracts (GSEs) were investigated in yeast cells harbouring defects in their antioxidant system (regarding the cellular growth and growth recovery from H2O2 insult). GSEs antioxidant activity was detected in wild-type and mutant strains Îcta1, Îgsh1 and Îoye2glr1, while pro-oxidant activity in Îsod1 cells was seen. Assessment of proliferation of prostate cancer PC3 and HBV-replicating HepG2 2.2.15 cells treated with GSEs has shown higher cytotoxicity of red grape seed extract (RW) than white grape seed extract (WW) subjective to dose and period of administration. No antiviral effect was detected by measuring the secreted virion particles in HepG2 2.2.15 cells treated with GSEs. The GSEs play a dual antioxidant/pro-oxidant role in vivo according with the cellular antioxidant system deficiencies and exhibit cytotoxic properties in PC3 and HepG2 2.2.15 cell lines, but no antiviral action against HBV.
Keywords
Antiviral actionhepatitis B “e” antigenPI3KFBSPCDHBsAgGSHFRAPHBeAgOYEGAEGSEGSSGHCCMAPKROSHepatitis B surface antigengallic acid equivalentsfetal bovine serumCytotoxicityGrape seed extractgrape seed extractsphosphatidylinositide 3-kinaseTotal polyphenolic contentYeastProgrammed cell deathHBVmitogen-activated protein kinaseHepatocellular carcinomaferric reducing antioxidant powerreduced glutathioneoxidised glutathioneReactive oxigen species
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Codruţa Ignea, Cristina Mihaela Dorobanţu, Christopher Paul Mintoff, Norica Branza-Nichita, Michael R. Ladomery, Panagiotis Kefalas, Veronica Sanda Chedea,