Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10539919 | Food Chemistry | 2013 | 8 Pages |
Abstract
Cultures of Escherichia coli 555 were grown at four levels of carvacrol (0-2Â mM) and the E. coli endo-metabolome was extracted and measured by 1H NMR spectroscopy. The results show that glucose concentration is going up with concentration of carvacrol and so do formate until the highest concentration is reached, from which point it suddenly decreases. This is interpreted as if the bacteria are increasingly unable to further metabolize glucose and as if the bacteria increasingly shifts with higher levels of carvacrol toward sugar fermentation as carbon source, until the level of carvacrol reaches a level (2.00Â mM), where the E. coli must give up. Additionally, the multivariate Principal Component Analysis suggests that the adaptation occurring at sub-lethal doses of carvacrol is different from that occurring at higher doses.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Gianfranco Picone, Luca Laghi, Fausto Gardini, Rosalba Lanciotti, Lorenzo Siroli, Francesco Capozzi,