| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10540337 | Food Chemistry | 2013 | 6 Pages |
Abstract
⺠Spinach aqueous extract suppressed antigen-induced degranulation of RBL-2H3 cells. ⺠Active substances in the extract were heat-stable and trypsin-resistant. ⺠The molecular weights of the active substances ranged from 500 Da to 14 kDa. ⺠The extract suppressed degranulation by decreased Ca2+ influx into the cells. ⺠Suppressed Ca2+ influx seems to result from decreased phosphorylation of Syk and PI3K.
Keywords
DNPSNETris-buffered saline containing 0.1% Tween 20RBL-2H3 cellPIP2NaPBPIP3FcεRIPI3KTBS-TMWCOHRPHSAPBSDMEMFBSIgEBSADulbecco’s modified Eagle medium[Ca2+]ihuman serum albuminbovine serum albuminSpinachstandard deviationImmunoglobulin Esodium phosphate bufferdinitrophenolfoetal bovine serumintracellular Ca2+ concentrationPhosphatidylinositol 3-kinasephosphatidylinositol 3,4,5-trisphosphatephosphatidylinositol 4,5-bisphosphateAnti-allergic activityDegranulationPhosphate-buffered salineMolecular Weight Cut OffHorseradish peroxidasehigh-affinity IgE receptor
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Momoko Ishida, Kosuke Nishi, Hisashi Watanabe, Takuya Sugahara,