| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10541032 | Food Chemistry | 2012 | 8 Pages |
Abstract
⺠This study presents a HPLC-Chip-MRM label-free method for the absolute quantification of γ-conglutin. ⺠Gamma-conglutin has a hypoglycaemic activity and it is a major lupin allergen. ⺠The standard addition approach is useful to evaluate the matrix effect. ⺠The standard addition approach is applied to the development of a label-free absolute quantification method in proteomics. ⺠The final output of the study is a robust external calibration curve for the quantification of the bioactive γ-conglutin.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Donatella Resta, Francesca Brambilla, Anna Arnoldi,