| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10541570 | Food Chemistry | 2011 | 4 Pages |
Abstract
⺠The Kunitz-type trypsin inhibitor from Acacia victoriae (Bentham) seeds (AvTI) is glycosylated to about 2.06%. ⺠Glutamate (13.25%), aspartate (10.33%), leucine (7.62%) and lysine (7.01%) were the major amino acids in AvTI. ⺠The dissociation constant (Ki) for the inhibition of bovine trypsin indicates a high affinity between AvTI and trypsin. ⺠The role of AvTI as a competitive inhibitor was confirmed by a double reciprocal plot.
Keywords
FPLCkDaRP-HPLCUPLCWattle seedKmappSDS–PAGEβ-mercaptoethanolperiodic acid-Schiffsodium dodecyl sulphate polyacrylamide gel electrophoresisTameMichaelis–Menten constantKineticsinhibition constantTrypsin inhibitorPASMolecular weightreversed phase-high performance liquid chromatographyfast-performance liquid chromatographyUltra-performance liquid chromatographykilodaltonsGlycoprotein
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
K.Y. Ee, J. Zhao, A. Rehman, S. Agboola,