Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10541804 | Food Chemistry | 2011 | 6 Pages |
Abstract
Penicillium oxalicum SO α-galactosidase demonstrated weak hydrolysing activity but a high rate of transglycosylation in the reaction with melibiose, where the major product was 6-α-galactosyl melibiose. The transfer ratio was 83.6% and was maintained over a long reaction time of 80 h. The molecular weight was estimated to be 124,000 by SDS-PAGE. The optimal pH was â¼3 and a stable pH, with a range of 2.4-9.5, was found. The optimal temperature was â¼60 °C and the activity was stable below 60 °C. With respect to acceptor specificity, mono-alcohols, sugar alcohols and sugars were poor acceptors, but the di-alcohol ethylene glycol and the tri-alcohol glycerin were good acceptors. The percentage of transglycosylation to glycerin increased up to 41.7%, as that to melibiose decreased, with the initial glycerin concentration of 40%. The production of α-d-galactosylglycerol was 293 mg for each gram of melibiose used by the enzymatic reaction.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Masahiro Kurakake, Youichirou Moriyama, Riku Sunouchi, Shinya Nakatani,