Apoptosis of human breast cancer cells induced by hemagglutinin from Phaseolus vulgaris cv. Legumi secchi

Article ID	Journal	Published Year	Pages	File Type
10543459	Food Chemistry	2011	8 Pages	PDF

Abstract

âº Hemagglutinin from seeds of Phaseolus vulgaris cultivar “Legumi secchi”. âº Antiproliferation of breast cancer MCF-7 cells. âº G2/M arrest/phosphatidylserine externalisation/mitochondrial membrane depolarisation. âº Death receptor-mediated: Fas ligands, caspase-8, BID, p53, caspase-9, Lamin A/C.

Keywords

Bcl-2 (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide)TRIS RNase JC-1 ECL HRP IC50 PVDF FITC PBS 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide BH3 Interacting Domain Death Agonist DNA MCF-7 breast cancer cells MTT SDS–PAGE EDTA Ethylenediaminetetraacetic acid deoxyribonucleic acid sodium dodecyl sulphate–polyacrylamide gel electrophoresis enhanced chemiluminescence Tris(hydroxymethyl)aminomethane Apoptosis ribonuclease Anti-tumor Phosphate buffered saline fluorescein isothiocyanate B-cell lymphoma 2 half maximal inhibitory concentration hemagglutinin HIV human immunodeficiency virus Horseradish peroxidase Propidium iodide Polyvinylidene fluoride BID