| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10543530 | Food Chemistry | 2011 | 6 Pages |
Abstract
⺠6-Amino-saccharin was used as a hapten and coupled to bovine serum albumin (BSA) for immunogen to produce specific polyclonal antibody (PcAb) for sodium saccharin. ⺠The PcAb was characterized by indirect ELISA and indirect competitive ELISA (icELISA) for its sensitivity and specificity. ⺠The ELISA conditions were optimized and an icELISA method for sodium saccharin was final developed with good sensitivity and specificity. ⺠The icELISA was applied to determination of sodium saccharin residues in food samples and the results were confirmed by HPLC method.
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Authors
Yu Wang, Zhen-Lin Xu, Yan-Yun Xie, Yuan-Xin Tian, Yu-Dong Shen, Glenn M. Young, Hong Wang, Hong-Tao Lei, Yuan-Ming Sun,