| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10546950 | Journal of the American Society for Mass Spectrometry | 2005 | 7 Pages |
Abstract
The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs+ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs]+ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] â 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Eduard Rogatsky, Harsha Jayatillake, Gayotri Goswami, Vlad Tomuta, Daniel Stein,