| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10547196 | Journal of the American Society for Mass Spectrometry | 2005 | 13 Pages |
Abstract
The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
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Authors
Chen Ren, Liwen Zhang, Michael A. Freitas, Kalpana Ghoshal, Mark R. Parthun, Samson T. Jacob,