Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10547780 | Journal of Chromatography A | 2011 | 7 Pages |
Abstract
There is a substantial amount of published literature on the bioavailability of various coffee components including the most abundant metabolites, caffeic and ferulic acids. Surprisingly, to date, the appearance of dimethoxycinnamic acid derivatives in humans has not been reported despite the fact that methylated form of catechol-type polyphenols could help maintain, modify or even improve their biological activities. This study reports an LC-MS method for the detection of dimethoxycinnamic acid in human plasma after treatment with an esterase. Liquid chromatography, including the combination of methanol and acetonitrile as organic eluent, was optimized to resolve all interferences and enable reliable detection and identification of 3,4-dimethoxycinnamic and 3,4-dimethoxy-dihydrocinnamic acids. In addition to the good mass accuracy achieved (better than 5Â ppm), tandem mass spectrometric and co-chromatography experiments further confirmed the identity of the compounds. The optimized method was applied to analyze samples obtained immediately, 1 and 10Â h after coffee ingestion. The results show that in particular 3,4-dimethoxycinnamic acid appears in high abundance (â¼380Â nM at 60Â min) in plasma upon coffee intake, indicating that it is important to consider these derivatives in future bioavailability and bioefficacy studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kornél Nagy, Karine Redeuil, Gary Williamson, Serge Rezzi, Fabiola Dionisi, Karin Longet, Frédéric Destaillats, Mathieu Renouf,