Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10547799 | Journal of Chromatography A | 2011 | 7 Pages |
Abstract
An on-line column-switching counter-current chromatography (CCC) with solid-phase trapping interphase is presented in this paper. The large volume injection is avoided using solid-phase trapping interphase. Thereby, totally different chemical composition biphasic solvent system can be utilized to enhance system orthogonality. In the present work, a 140Â mL-capacity CCC instrument was used in the first dimension, and a parallel three-coil CCC centrifuge (40Â mL each coil) was used in the second dimensional separation allowing three injections at the same time. With biphasic solvent system composed of n-hexane: ethyl acetate: methanol: water (1:1:1:1, v/v), five well-separated fractions were obtained in the first dimension. Two fractions were online concentrated and further separated in the second dimension with solvent system composed of methyl tert-butyl ether: acetonitrile: water (2:2:3, v/v), where trifluoroacetic acid (10Â mM) was added to the upper organic phase as a retainer and triethylamine (10Â mM) to the aqueous mobile phase as an eluter. Four hydroxyanthraquinones were successfully separated and purified from Chinese medicinal plant Rheum officinale in only one step.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ruilin Hu, Xiaojing Dai, Xia Xu, Cuirong Sun, Yuanjiang Pan,