Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10547804 | Journal of Chromatography A | 2011 | 6 Pages |
Abstract
Reversed phase HPLC (RP-HPLC) and high performance countercurrent chromatography (HPCCC) were compared for the pilot scale purification of two semi-synthetic spinosyns, spinetoram-J and spinetoram-L, the major components of the commercial insecticide spinetoram. Two, independently performed, 1 kg, purification campaigns were compared. Each method resulted in the isolation of both components at a purity of >97% and yields for spinetoram-J and spinetoram-L of >93% and â¥63% of theoretical, respectively. The HPCCC process produced a 2-fold higher throughput and consumed approximately 70% less solvent than preparative scale RP-HPLC, the volume of product containing fractions from HPCCC amounted to 7% of that produced by HPLC and so required much less post-run processing.
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Authors
Carl DeAmicis, Neil A. Edwards, Michael B. Giles, Guy H. Harris, Peter Hewitson, Lee Janaway, Svetlana Ignatova,