Article ID Journal Published Year Pages File Type
10547854 Journal of Chromatography A 2005 5 Pages PDF
Abstract
High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of geniposide from Gardenia jasminoides Ellis. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). According to the above solvent system, preparative HSCCC was successfully performed with the optimal solvent system composed of ethyl acetate-n-butanol-water (2:1.5:3, v/v/v) yielding 389 mg of geniposide at over 98% purity from 1 g of the partially purified extract with 38.9% recovery in a one-step separation.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , ,