Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10547854 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of geniposide from Gardenia jasminoides Ellis. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). According to the above solvent system, preparative HSCCC was successfully performed with the optimal solvent system composed of ethyl acetate-n-butanol-water (2:1.5:3, v/v/v) yielding 389Â mg of geniposide at over 98% purity from 1Â g of the partially purified extract with 38.9% recovery in a one-step separation.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tingting Zhou, Guorong Fan, Zhanying Hong, Yifeng Chai, Yutian Wu,