| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10547891 | Journal of Chromatography A | 2005 | 7 Pages |
Abstract
Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for '1:1' fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within ±5% when compared with more traditional post-purification analysis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ding Yuan, Chris Petersen, Matthew Rynd, James Mainquist, Andrew Meyer, John J. Isbell,