Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10547893 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of trans-3,5,4â²-trihydroxystilbene-4â²-O-β-d-glucopyranoside (compound 1) and (+)catechin (compound 2) from Rheum tanguticum Maxim. ex Balf. by stepwise elution with a pair of two-phase solvent system composed of ethyl acetate-ethanol-water (25:1:25, v/v) and (5:1:5, v/v), and stepwise increasing the flow-rate of the mobile phase from 0.8 to 2.0 ml minâ1 after 5 h. The preparative HSCCC separation was performed on 250 mg of crude extract yielding pure compound 1 (10.2 mg) and compound 2 (26.7 mg) all at purities of over 96% in a single run. The structures of the two compounds have been elucidated by means of spectroscopic methods including MS and 1H, 13C nuclear magnetic resonance spectroscopy.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Jin, Peng-Fei Tu,