| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10547896 | Journal of Chromatography A | 2005 | 4 Pages |
Abstract
A high-performance liquid chromatography-diode array detection (HPLC-DAD) assay is described for the detection and quantification of the secreted Beauveria brongniartii metabolite oosporein from potato tubers. Analyte recovery was achieved with a Britton-Robinson buffer system at pH 5.5 diluted with methanol 3:7 (v/v) (BR5.5-MeOH). An internal standard protocol using 2-iodobenzoic acid was established to minimize analytical error. The resulting assay, using a binary solvent gradient with acidic modifiers and detecting the metabolite at 287Â nm, showed a limit of detection (LOD) of 2.4Â mg oosporein/kg potato tubers. The oosporein content of potato tuber samples obtained from a field trial using the biological pest control B. brongniartii formulation Melocont-Pilzgerste in up to five-fold higher doses (250Â kg Melocont-Pilzgerste/ha) as recommended per year was found to be below the established LOD.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Christoph Seger, Tobias Längle, Barbara Pernfuss, Hermann Stuppner, Hermann Strasser,