Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10548013 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
Acebutolol [N-{3-acetyl-4-[(2-hydroxy-3-(isopropylamino)propoxy]phenyl} butanamide] is a cardioselective β-blocker with a potent antihypertensive and antiarrhythmic effect. The optimised operational system of electrolytes for the newly developed ITP separation of acebutolol consisted of 10 mM potassium acetate +10 mM acetic acid (pH 4.65) as the leading electrolyte and 10 mM β-alanine with pH â4 (adjusted with acetic acid) as the terminating electrolyte. The driving and detection currents were 75 and 20 μA, respectively and the analysis took â13 min. Under these conditions the effective mobility of acebutolol was determined as 20.7 Ã 10â9 m2 Vâ1 sâ1. The calibration dependence was rectilinear in the range 0.14-1.4 mg mlâ1 of acebutolol base (r = 0.9995); relative standard deviation (RSD) values were 1.1% and 1.2% (n = 6) when determining 0.42 and 0.98 mg mlâ1 of acebutolol in a pure standard solution. The method, with the limit of detection (LOD) of 0.04 mg mlâ1 and limit of quantification (LOQ) of 0.12 mg mlâ1, was applied to the assay of acebutolol in Sectral tablets, Acecor tablets, Apo-acebutol tablets (nominal content 400 mg of acebutolol per tablet) and Acebirex tablets (nominal content 200 mg of acebutolol per tablet) with RSD = 0.7-1.7% (n = 6). No interference from any excipients present in the tablets was observed. The recoveries ranged from 98.8% to 102.4% as found by the standard addition technique.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marie PospÃÅ¡ilová, Andrea KavalÃrová, Miroslav PoláÅ¡ek,