Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10548084 | Journal of Chromatography A | 2005 | 8 Pages |
Abstract
A method for detection of semicarbazide (SEM) in baby food was validated. SEM was extracted with hydrochloric acid and derivatised with 2-nitrobenzaldehyde, using [15N2,13C] semicarbazide as internal standard. The extract was neutralised, purified on a solid phase extraction cartridge and SEM was determined by reversed phase LC-MS-MS. Linearity was demonstrated in the ranges from 0.1 ng mlâ1 to 1 ng mlâ1 and from 2 ng mlâ1 to 80 ng mlâ1. Matrix effects were non significant for meat-based and significant for apple and rice-based baby foods, in both ranges. Mean recoveries ranged from 87.8% to 107.2% with relative standard deviation from 0.2% to 9.1%, considering both ranges. Limits of detection and quantification were 0.1 μg kgâ1 and 0.25 μg kgâ1, respectively. The results of the validation process demonstrated the method suitability for use in food control.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Scheilla V.C. de Souza, Roberto G. Junqueira, Richard Ginn,