Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10548090 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
A high-performance thin layer chromatographic (HPTLC) method for the rapid and simple quantification of the four major anthraquinone derivatives i.e. physcion, chrysophanol, emodin and chrysophanol glycoside in Rheum emodi is described. HPTLC of anthraquinone derivatives was performed on pre-coated RP-18 F254S HPTLC plates. For achieving good separation, the mobile phase of methanol-water-formic acid (80:19:1, v/v/v) was used. The densitometric determination of anthraquinone derivatives was carried out at 445Â nm in reflection/absorption mode. The calibration curves were linear in the range of 20-100Â ng for physcion, 80-400Â ng for chrysophanol and emodin, and 200-1000Â ng for chrysophanol glycoside. The method was found to be reproducible and convenient for quantitative analysis of anthraquinone derivatives in the methanolic extract of rhizomes of R. emodi collected from three different locations of Western Himalaya, India.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Narendra P. Singh, Ajai P. Gupta, Arun K. Sinha, Paramvir S. Ahuja,