Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10548248 | Journal of Chromatography A | 2005 | 5 Pages |
Abstract
A novel, rapid and continuous on-line concentration approach based on dynamic pH junction for the analysis of trimethoprim (TMP) and sulfamethoxazole (SMZ) by microfluidic capillary electrophoresis (CE) combined with flow injection analysis is developed in this paper. Stacking is due to decreases in the velocity of analytes when migrating from the low-pH sample zone (sample was dissolved in 50Â mM HCl) to a relatively high-pH buffer (30Â mM phosphate buffer, pH 8.5) filled in the capillary. This results in 2.9-4.7-fold improvement in concentration sensitivity relative to conventional capillary electrophoresis methods. The separation could be achieved within 2Â min and sample throughput rate can reach up to 38Â hâ1.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Liuyin Fan, Lihong Liu, Hongli Chen, Xingguo Chen, Zhide Hu,