Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550017 | Journal of Chromatography B | 2019 | 6 Pages |
Abstract
A high performance liquid chromatography coupled with electrospray ionization/mass spectrometry method was developed for the determination of adenosine 5â²-monophosphate (AMP), adenosine 5â²-diphosphate (ADP), and adenosine 5â²-triphosphate (ATP) in the extract of HepG-2 cells. The chromatographic conditions were optimized by using porous graphitic carbon as the stationary phase for the retention and separation of the AMP, ADP and ATP. Negative-ion mode ESI-MS in basic mobile phase was applied to improve the method sensitivity. An external calibration method with linear ranges from 0.22 to 57.80 μM for AMP, from 0.59 to 117.37 μM for ADP, and from 0.49 to 98.81 μM for ATP was used for quantitative analysis. The levels of ATP, ADP, and AMP in HepG-2 cells treated with benzo[a]pyrene with different time periods were determined. Total adenine nucleotides and the energy charge potential were calculated for the investigation of the effect of benzo[a]pyrene on cell energy metabolism.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jun Wang, Tao Lin, Jiaping Lai, Zongwei Cai, M.S. Yang,