Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550032 | Journal of Chromatography B | 2009 | 5 Pages |
Abstract
A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 μg/mL) in human plasma (r2 = 0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3% and 107.6%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kang Choon Lee, Young Goo Chun, Insoo Kim, Beom Soo Shin, Eun-Seok Park, Sun Dong Yoo, Yu Seok Youn,