Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550042 | Journal of Chromatography B | 2005 | 6 Pages |
Abstract
Mianserin is a tetracyclic antidepressant drug and administered as racemate of R (â) and S (+) mianserin hydrochloride in a dose of 30-90Â mg/day in divided doses. Liquid chromatography-mass spectroscopy (LC-MS) is a tool, which is widely used for determination of drug and their metabolites in biological fluids because of its high sensitivity and precision. Here we describe a liquid chromatography mass spectroscopy method for simultaneous determination of mianserin and its metabolite, N-desmethylmianserin, from human plasma using a liquid-liquid extraction with hexane:isoamylalcohol (98:2) and back extraction with 0.005Â M formic acid solution. This method is specific and linear over the concentration range of 1.00-60.00Â ng/ml for mianserin and 0.50-14.00Â ng/ml for N-desmethylmianserin in human plasma. The lowest limits of quantification (LLQ) is 1.00Â ng/ml for mianserin and 0.50Â ng/ml for N-desmethylmianserin. Intraday and interday precision (%C.V.) is <10% for both mianserin and N-desmethylmianserin. The accuracy ranges from 94.44 to 112.33% for mianserin and 91.85-100.13% for N-desmethylmianserin. The stability studies showed that mianserin and N-desmethylmianserin in human plasma are stable during short-term period for sample preparation and analysis. The method was used to assay mianserin and its metabolite, N-desmethylmianserin, in human plasma samples obtained from subjects who had been given an oral tablet of 30Â mg of mianserin.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bhupendrasinh Chauhan, Shubha Rani, Swati Guttikar, Anjali Zope, Nimisha Jadon, Harish Padh,