Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550044 | Journal of Chromatography B | 2005 | 16 Pages |
Abstract
We constructed a novel database of the proteome of DLD-1 colon cancer cells by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of fluorescence-labeled proteins followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis. The database consists of 258 functionally categorized proteins corresponding to 314 protein spots. The majority of the proteins are oxidoreductases, cytoskeletal proteins and nucleic acid binding proteins. Phosphatase treatment showed that 28% of the protein spots on the gel are phosphorylated, and mass spectrometric analysis identified 21 of them. Proteins of DLD-1 cells and of laser-microdissected colon cancer tissues showed similar distribution on 2D gels, suggesting the utility of our database for clinical proteomics.
Keywords
MALDI-TOFN-tosyl-l-phenylalanine chloromethyl ketoneDTT2D-PAGETPCKDHB2D-DIGEcIAPIPGimmobilized pH gradientmatrix-assisted laser desorption/ionization time-of-flight mass spectrometrytwo-dimensional gel electrophoresistwo-dimensional polyacrylamide gel electrophoresisdihydroxybenzoic aciddithiothreitolCalf intestine alkaline phosphataseColon cancerProteome
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yasuharu Mori, Tadashi Kondo, Tesshi Yamada, Akihiko Tsuchida, Tetsuya Aoki, Setsuo Hirohashi,