Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550053 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
A rapid and specific HPLC method has been developed and validated for simultaneous determination of clobazam, the anticonvulsant agent, and its major metabolite in human plasma. The sample preparation was a liquid-liquid extraction with tuloene yielding almost near 100% recoveries of two compounds. Chromatographic separation was achieved with a Chromolith⢠Performance RP-18e 100 mm Ã 4.6 mm column, using a mixture of a phosphate buffer (pH 3.5; 10 mM)-acetonitrile (70:30, v/v), in isocratic mode at 2 ml/min at a detection wave-length of 228 nm. The calibration curves were linear (r2 > 0.998) in the concentration range of 5-450 ng mlâ1. The lower limit of quantification was 5 ng mlâ1 for two compounds studied. The within- and between-day precisions in the measurement of QC samples at four tested concentrations were in the range of 0.89-9.1% and 2.1-10.1% R.S.D., respectively. The developed procedure was applied to assess the pharmacokinetics of clobazam and its major metabolite following administration of a single 10 mg oral dose of clobazam to healthy volunteers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mohammadreza Rouini, Yalda H. Ardakani, Lida Hakemi, Maryam Mokhberi, Gheise Badri,