Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550089 | Journal of Chromatography B | 2005 | 8 Pages |
Abstract
An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C8 analytical column. The mobile phase consisted of methanol-water (70:30; v/v)Â +Â 12Â mM formic acid. The method had a chromatographic total run time of 3.5Â min and was linear within the range 1.00-500Â ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ney Carter do C. Borges, Gustavo D. Mendes, Rafael E. Barrientos-Astigarraga, Paulo Galvinas, Celso H. Oliveira, Gilberto De Nucci,