Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550208 | Journal of Chromatography B | 2005 | 4 Pages |
Abstract
The determination of theanine has been performed by micellar electrokinetic capillary chromatography (MECC) using 2,4-dinitrofluorobenzene (DNFB) as a derivative reagent. To achieve the separation, a fused-silica capillary column was used with a borax buffer at 0.03 mol/L pH 9.8 (containing Brij35 and isopropanol) at 17 °C with detection wave length at 360 nm. The factors affecting the efficiency of the sample separation were examined simultaneously. A 40-min reaction at 35 °C between l-glutamate and ethylamine (with Tris-HCl buffer, pH 7.5) was investigated using the theanine synthetase from budding tea seeds. A novel method for the analysis of theanine synthetase activity based on MECC was established. The method shows mean recovery ranged from 87.1 to 105.3% and linearity ranged from 0.2 to 5.0 mmol/L.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ping Li, Xiao-Chun Wan, Zheng-Zhu Zhang, Jian Li, Zuo-Jun Shen,