| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10550211 | Journal of Chromatography B | 2005 | 7 Pages |
Abstract
An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for pre-column derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6% for FB1 and 95.3% for AP1. The limit of quantification (LOQ) in swine liver was 75 μg/kg for FB1 and 42 μg/kg for AP1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Giampiero Pagliuca, Elisa Zironi, Alberto Ceccolini, Riccardo Matera, Gian Paolo Serrazanetti, Andrea Piva,