| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10550337 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
The enhanced green fluorescent protein (EGFP) was over-expressed in Escherichia coli as inclusion bodies to increase its quantity and to facilitate its purification. Insoluble EGFP has been purified on Q Hyper Z matrix by expanded bed adsorption after solubilization in 8Â M urea. The adsorption was made in expanded bed mode to avoid centrifugation. EBA-column refolding was done by elimination of urea and elution with NaCl. The EGFP was obtained as a highly purified soluble form with similar behavior in fluorescence and electrophoresis as native EGFP.
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Authors
C. Cabanne, A.M. Noubhani, A. Hocquellet, F. Dole, W. Dieryck, X. Santarelli,